I am a senior postdoctoral research associate with ten years’ experience of elucidating disease mechanism and developing novel drug, protein, viral and stem cell therapies.
From 2018 I am a senior post doctoral research associate in the laboratories of Dr Stephen White at Manchester Metropolitan University and Professor Martin Humphries in the Wellcome Trust Centre for Cell-Matrix research at the University of Manchester. The aim of my current research is to define the mechanisms of normal and pathological force sensing by endothelial cell adhesion complexes. Using this information, we will determine the consequences of targeted interventions on endothelial detachment as a potential therapeutic strategy for atherosclerosis.
2006 Trinity College, University of Oxford, Molecular and Cellular Biochemistry (MBiochem)
2012 University of Manchester, PhD
2012 - 2015 University of Manchester – Postdoctoral Research Associate
2016- 2018 Manchester Metropolitan University – Senior Postdoctoral Research Associate
I've worked on understanding disease mechanisms and developed novel drug, protein, viral and stem cell therapies for: neurodegenerative disorders, lysosomal storage disorders, mucopolysaccharidosis, age-related macular degeneration, ageing, vascular disorders, diabetes, systemic lupus erythematosus and atherosclerosis.
Research Techniques
Cell Biology
Primary cell isolation and culture, including endothelial stem cells and bone marrow. Tissue culture of multiple different cell lines. Development of cell based assays for cell viability, reactive oxygen species assays, metabolic function assays, and environmental stress (including metal ions, oxidative stress and inflammation). Use of scratch, migration and Matrigel assays for assessment of angiogenesis and endothelial cell function. Culture of cells on novel scaffolds to assess the role of the matrix composition and stiffness on cell functions. Production and purification of extracellular vesicles (microvesicles and exosomes). Production and titration of high titre lentiviral vector by QPCR, P24 ELISA and flow cytometry. Transduction and transfection of primary cells and cell lines with plasmids, retroviral and lentiviral vector.
Large data set analysis
Transcriptomic analysis with pathway analysis. Multivariate comparison of environmental, genetic and gene expression data.
Protein and matrix
Production of recombinant protein in bacterial and human cells and purification by FPLC and HPLC. ELISA, western blot, enzyme assays, glycosaminoglycan assays (Blyscan, AMAC) and functional complement assays. ICP-MS metal ion analysis.
Molecular biology
Plasmid and genomic DNA and RNA (mRNA, miRNA rRNA) isolation, PCR, QPCR, southern blot, gel electrophoresis, genotyping, cloning, digestion, ligation, transformation and sequencing. Production of novel expression and reporter constructs.
Mouse in vivo techniques
Bone marrow transplant including models of neurodegenerative lysosomal storage disorders, tail vein bleed, intravenous, intraperitoneal and subcutaneous injections, PET scanning. Mouse behavioral analysis to assess neurodegeneration: open field test, elevated plus maze, hanging bar and inverted screen. Breeding and colony management.
Human tissue
Human eyes, urine, saliva, blood and cord blood processing and record maintenance in accordance with HTA. Statistical analysis of patient data.
Tissue analysis
Frozen or fixed tissue sectioning of brain, eye and other tissues. Histological stains, antibody stains, confocal and laser capture microdissection.
AM. Mahmoud, FL. Wilkinson, EM. McCarthy, D. Moreno-Martinez, A. Langford-Smith, et al. M. Romero, J. Duarte, MY. Alexander. (2017). Endothelial microparticles prevent lipid-induced endothelial damage via Akt/eNOS signaling and reduced oxidative stress. The FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 31(10), pp.4636-4648.
RJ. Holley, SM. Ellison, D. Fil, C. O'Leary, J. McDermott, et al. N. Senthivel, A. Langford-Smith, FL. Wilkinson, Z. D'Souza, H. Parker, A. Liao, S. Rowlston, H. Gleitz, S. Kan, P. Dickson, BW. Bigger. Macrophage enzyme and reduced inflammation drive brain correction of mucopolysaccharidosis IIIB by stem cell gene therapy. Brain. 141(1), pp.99-116.
C. Martins, H. Hulkova, L. Dridi, V. Dormoy-Raclet, L. Grigoryeva, et al. Y. Choi, A. Langford-Smith, FL. Wilkinson, K. Ohmi, G. DiCristo, E. Hamel, J. Ausseil, D. Cheillan, A. Moreau, E. Svobodova, Z. Hajkova, M. Tesarova, H. Hansikova, BW. Bigger, M. Hrebicek, AV. Pshezhetsky. (2015). Corrigendum. Brain. 138(Pt 7), pp.e366-e366.
C. Martins, H. Hůlková, L. Dridi, V. Dormoy-Raclet, L. Grigoryeva, et al. Y. Choi, A. Langford-Smith, FL. Wilkinson, K. Ohmi, G. DiCristo, E. Hamel, J. Ausseil, D. Cheillan, A. Moreau, E. Svobodová, Z. Hájková, M. Tesařová, H. Hansíková, BW. Bigger, M. Hrebícek, AV. Pshezhetsky. (2015). Neuroinflammation, mitochondrial defects and neurodegeneration in mucopolysaccharidosis III type C mouse model. Brain : a journal of neurology. 138(Pt 2), pp.336-355.
A. Langford-Smith, FL. Wilkinson, KJ. Langford-Smith, RJ. Holley, A. Sergijenko, et al. SJ. Howe, WR. Bennett, SA. Jones, JE. Wraith, CL. Merry, RF. Wynn, BW. Bigger. (2012). Hematopoietic Stem Cell and Gene Therapy Corrects Primary Neuropathology and Behavior in Mucopolysaccharidosis IIIA Mice. Mol Ther. 20(8), pp.1610-1621.
A. Langford-Smith, FL. Wilkinson, KJ. Langford-Smith, RJ. Holley, A. Sergijenko, et al. SJ. Howe, WR. Bennett, SA. Jones, J. Wraith, CL. Merry, RF. Wynn, BW. Bigger. (2012). Hematopoietic stem cell and gene therapy corrects primary neuropathology and behavior in mucopolysaccharidosis IIIA mice. Mol Ther. 20(8), pp.1610-1621.
A. Langford-Smith, M. Malinowska, KJ. Langford-Smith, G. Wegrzyn, S. Jones, et al. R. Wynn, JE. Wraith, FL. Wilkinson, BW. Bigger. (2011). Hyperactive behaviour in the mouse model of mucopolysaccharidosis IIIB in the open field and home cage environments. Genes Brain Behav. 10(6), pp.673-682.
S. Satta, M. McElroy, A. Langford-Smith, G. Ferris, J. Teasdale, et al. Y. Kim, G. Niccoli, A. Tanjeko, J. Serré, G. Hazell, G. Sala-Newby, P. Wang, J. Johnson, M. Humphries, G. Gayan-Ramirez, P. Libby, F. Crea, H. Degens, F. Gijsen, T. Johnson, A. Keshmiri, Y. Alexander, A. Newby, S. White. (2019). 143 A pivotal role for NRF2 in endothelial detachment–implications for endothelial erosion of stenotic plaques. In: Acute Coronary Syndromes. Manchester, ENGLAND, 3/6/2019. pp.A118-A118.
Y. Alexander, AW. Langford-Smith, A. Hassan, N. Edwards, FL. Wilkinson (2019). Endothelial Dysfunction in the Diabetic Patient and the Contribution by Circulating Endothelial Colony Forming Cells. In: JOURNAL OF VASCULAR RESEARCH. Maastricht, NETHERLANDS, 15/4/2019. pp.3-3.
S. Satta, M. Mcelroy, AW. Langford-Smith, G. Ferris, J. Teasdale, et al. Y. Kim, G. Niccoli, TT. Ajime, G. Ghayan-Ramirez, J. Serre, G. Hazell, GS. Newby, P. Wang, J. Johnson, M. Humphries, F. Crea, H. Degens, F. Gijsen, T. Johnson, A. Keshmiri, Y. Alexander, A. Newby, S. White. (2019). High-Level Nrf2 Activation Promotes Endothelial Detachment - Implications for Acute Coronary Syndromes Triggered by Endothelial Erosion of Plaques. In: JOURNAL OF VASCULAR RESEARCH. Maastricht, NETHERLANDS, 15/4/2019. pp.71-71.
N. Edwards, AW. Langford-Smith, S. Tandel, B. Parker, I. Bruce, et al. J. Reynolds, Y. Alexander, E. McCarthy, FL. Wilkinson. (2019). Improving Stratification of Cardiovascular Risk in Systemic Lupus Erythematosus using Endothelial Microvesicles as Novel Biomarkers. In: JOURNAL OF VASCULAR RESEARCH. Maastricht, NETHERLANDS, 4/2019. pp.32-33.
N. Edwards, AWW. Langford-Smith, M. Floren, A. Hasan, R. Weston, et al. ST. Rashid, A. Boulton, F. Bowling, W. Tan, FL. Wilkinson, MY. Alexander. (2019). Mitochondrial Hyperactivity, Impaired Matrix Adhesion and Functional Activity in Endothelial Colony Forming Cells Isolated from Patients with Diabetic Foot Ulcers. In: CARDIOVASCULAR DRUGS AND THERAPY. pp.273-273.
AWW. Langford-Smith, M. Floren, A. Hasan, R. Weston, N. Edwards, et al. ST. Rashid, A. Boulton, F. Bowling, W. Tan, FL. Wilkinson, MY. Alexander. (2018). Altered matrix adhesion, impaired function and mitochondrial hyperactivity in endothelial colony-forming cells isolated from patients with diabetic foot ulcers. In: INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY. Manchester, ENGLAND, 21/7/2018. pp.A7-A8.
AWW. Langford-Smith, M. Floren, T. Wei, A. Hasan, R. Weston, et al. N. Edwards, FL. Wilkinson, Y. Alexander, ST. Rashid, F. Bowling, A. Boulton. (2018). 108 Altered matrix adhesion, impaired function and mitochondrial hyperactivity in endothelial colony forming cells isolated from patients with diabetic foot ulcers. In: Basic Science. Manchester, ENGLAND, 4/6/2018. pp.A85-A85.
N. Edwards, AWW. Langford-Smith, B. Parker, I. Bruce, JA. Reynolds, et al. Y. Alexander, E. McCarthy, FL. Wilkinson. (2018). 147 QRISK3 improves identification of endothelial dysfunction and cardiovascular disease risk in patients with systemic lupus erythematosus. In: Basic Science. Manchester, ENGLAND, 4/6/2018. pp.A106-A106.
S. Skeoch, F. Wilkinson, A. Langford-Smith, J. Waterton, IN. Bruce, et al. Y. Alexander. (2018). SERUM OSTEOPONTIN LEVELS ARE ASSOCIATED WITH THE SUBCLINICAL CARDIOVASCULAR DISEASE IN PATIENTS WITH RHEUMATOID ARTHRITIS. In: RHEUMATOLOGY. Liverpool, ENGLAND, 1/5/2018.
S. Skeoch, A. Langford-Smith, F. Wilkinson, B. Parker, J. Waterton, et al. IN. Bruce, Y. Alexander. (2018). LEVELS OF CIRCULATING ENDOTHELIAL DERIVED MICROVESICLES IN PATIENTS WITH RHEUMATOID ARTHRITIS. In: RHEUMATOLOGY. Liverpool, ENGLAND, 1/5/2018.
N. Payton, A. Langford-Smith, R. Weston, A. Hasan, A. Jones, et al. A. Boulton, F. Bowling, ST. Rashid, F. Wilkinson, MY. Alexander. (2017). The Therapeutic Effects of a Novel Glycomimetic on the Function of Endothelial Colony Forming Progenitor Cells Isolated From Patients With Diabetic Foot Ulcers. In: JOURNAL OF VASCULAR RESEARCH. Geneva, SWITZERLAND, 29/5/2017. pp.52-52.
PN. Bishop, S. McHarg, N. Brace, AWW. Langford-Smith, R. Unwin, et al. R. Perveen, GC. Black, A. Day, SJ. Clark. (2017). Expression of complement and other inflammatory pathway genes is co-ordinated in the human RPE cell transcriptome. In: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE. Baltimore, MD, 7/5/2017.
R. Holley, S. Ellison, D. Fil, C. O'Leary, J. McDermott, et al. M. Senthivel, A. Langford-smith, F. Wilkinson, P. Dickson, B. Bigger. (2017). Haematopoietic stem cell gene therapy corrects the brain in mucopolysaccharidosis type IIIB. In: HUMAN GENE THERAPY. Royal Welsh Coll Mus & Drama, Cardiff, WALES, 19/4/2017. pp.A12-A12.
B. Bigger, S. Ellison, D. Fil, C. O'leary, J. McDermott, et al. N. Senthivel, A. Langford-Smith, F. Wilkinson, S-H. Kan, P. Dickson, R. Holley. (2017). Neurological correction of mucopolysaccharidosis type IIIB mice by haematopoietic stem cell gene therapy. In: Molecular Genetics and Metabolism. San Diego, CA, 13/2/2017. pp.S28-S28.
SM. Ellison, R. Holley, D. Fil, J. Mc Dermott, N. Senthivel, et al. A. Langford-Smith, F. Wilkinson, S. Jones, R. Wynn, S-H. Kan, P. Dickson, B. Bigger. (2016). Neurological Correction of Mucopolysaccharidosis IIIB Mice by Haematopoietic Stem Cell Gene Therapy. In: Molecular Therapy. Washington, DC, 4/5/2016. pp.S146-S146.
AJ. Day, A. Langford-Smith, V. Tilakaratna, L. Logunova, P. Lythgoe, et al. SJ. Clark, PN. Bishop. (2016). Age-related accumulation of metal ions in Bruch's membrane: implications for AMD. In: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE. Seattle, WA, 1/5/2016.
A. Langford-Smith, V. Tilakaratna, L. Logunova, PR. Lythgoe, SJ. Clark, et al. PN. Bishop, AJ. Day. (2016). Age-related accumulation of metal ions in human retinal matrix: implications for age-related macular degeneration. In: INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY. Chester Univ, Chester, ENGLAND, 4/4/2016. pp.A19-A19.
SM. Ellison, A. Sergijenko, A. Langford-Smith, K. Langford-Smith, R. Wynn, et al. F. Wilkinson, B. Bigger. (2015). Pre-clinical workup of lentiviral mediated stem cell gene therapy for mucopolysaccharidosis type IIIA. In: HUMAN GENE THERAPY. Helsinki, FINLAND, 17/9/2015. pp.A16-A16.
S. Ellison, A. Sergijenko, A. Langford-smith, K. Langford-smith, R. Wynn, et al. F. Wilkinson, B. Bigger. (2015). Pre-clinical workup of lentiviral mediated stem cell gene therapy for mucopolysaccharidosis type IIIA. In: HUMAN GENE THERAPY. Glasgow, SCOTLAND, 9/6/2015. pp.A27-A27.
SM. Ellison, A. Sergijenko, A. Langford-Smith, K. Langford-Smith, F. Wilkinson, et al. R. Wynn, B. Bigger. (2015). Pre-clinical workup of lentiviral mediated stem cell gene therapy for mucopolysaccharidosis type IIIA. In: Molecular Genetics and Metabolism. Orlando, FL, 9/2/2015. pp.S41-S41.
A. Pshezhetsky, C. Martins, H. Hůlková, L. Dridi, L. Grigoryeva, et al. A. Langford-Smith, F. Wilkinson, K. Ohmi, J. Ausseil, ES. Svobodová, Z. Hájková, M. Tesařová, H. Hansíková, B. Bigger, M. Hrebícek. (2015). Brain disease in mucopolysaccharidosis III C mouse: Neuroinflammation, mitochondrial defects and neurodegeneration. In: Molecular Genetics and Metabolism. Orlando, FL, 9/2/2015. pp.S97-S97.
S. Ellison, A. Sergijenko, A. Langford-Smith, K. Langford-Smith, E. Wraith, et al. R. Wynn, F. Wilkinson, B. Bigger. (2014). Development of Lentiviral Mediated Stem Cell Gene Therapy for Mucopolysaccharidosis Type IIIA. In: MOLECULAR THERAPY. Washington, DC, 21/5/2014. pp.S8-S8.
A. Sergijenko, A. Langford-Smith, AY. Liao, CE. Johnson, J. McDermott, et al. FL. Wilkinson, KJ. Langford-Smith, CLR. Merry, SA. Jones, JE. Wraith, RF. Wynn, BW. Bigger. (2013). Myeloid driven stem cell gene therapy corrects a mouse model of Mucopolysaccharidiosis IIIA. In: HUMAN GENE THERAPY. Madrid, SPAIN, 25/10/2013. pp.A134-A134.
T. Keenan, S. Clark, A. Langford-Smith, C. Pickford, R. Holley, et al. C. Merry, A. Day, P. Bishop. (2013). Age-related changes to glycosaminoglycans in human Bruch's membrane may contribute to immune dysregulation in AMD. In: Molecular Immunology. Jena, GERMANY, 17/8/2013. pp.280-280.
JH. McDermott, A. Langford-Smith, A. Sergijenko, KJ. Langford-Smith, A. Liao, et al. SA. Jones, JE. Wraith, RF. Wynn, FW. Wilkinson, BW. Bigger. (2013). Lentiviral Vector Mediated Haematopoietic Stem Cell Gene Therapy for the Treatment of Lysosomal Disease. In: JOURNAL OF PATHOLOGY. Univ Edinburgh, Div Pathol, Edinburgh, SCOTLAND, 18/6/2013. pp.20-20.
A. Sergijenko, A. Langford-Smith, AY. Liao, C. Pickford, J. McDermott, et al. K. Langford-Smith, C. Merry, S. Jones, E. Wraith, R. Wynn, F. Wilkinson, B. Bigger. (2013). Myeloid driven stem cell gene therapy corrects a mouse model of Mucopolysaccharidiosis IIIA. In: Molecular Genetics and Metabolism. Orlando, FL, 12/2/2013. pp.S83-S83.
JH. McDermott, A. Sergijenko, A. Langford-Smith, A. Liao, CE. Pickford, et al. G. Nowinski, KJ. Langford-Smith, CLR. Merry, SA. Jones, JE. Wraith, RF. Wynn, FW. Wilkinson, BW. Bigger. (2013). Myeloid Driven Stem Cell Gene Therapy Corrects the Neuropathology of Lysosomal Disease. In: JOURNAL OF PATHOLOGY. Univ Med Ctr, Dept Pathol, Utrecht, NETHERLANDS, 8/1/2013. pp.S30-S30.
A. Langford-Smith, A. Sergijenko, F. Wilkinson, K. Langford-Smith, AY. Liao, et al. S. Jones, JE. Wraith, R. Wynn, B. Bigger. (2012). Lentiviral SGSH expression from the human CD11b promoter in transplanted haematopoietic stem cells fully corrects behaviour and neuropathology of Mucopolysaccharidosis IIIA mice. In: HUMAN GENE THERAPY. Versailles, FRANCE, 25/10/2012. pp.A50-A50.
A. Langford-Smith, A. Sergijenko, FL. Wilkinson, KJ. Langford-Smith, A. Liao, et al. SA. Jones, JE. Wraith, RF. Wynn, BW. Bigger. (2012). LENTIVIRAL SGSH EXPRESSION FROM THE HUMAN CD11B PROMOTER IN TRANSPLANTED HAEMATOPOIETIC STEM CELLS FULLY CORRECTS BEHAVIOUR AND NEUROPATHOLOGY OF MUCOPOLYSACCHARIDOSIS IIIA MICE. In: JOURNAL OF INHERITED METABOLIC DISEASE. pp.S21-S21.
FL. Wilkinson, RF. Holley, KJ. Langford-Smith, S. Badrinath, A. Langford-Smith, et al. JD. Cooper, SA. Jones, JE. Wraith, RF. Wynn, CLR. Merry, BW. Bigger. (2012). NEUROPATHOLOGICAL CHANGES ARE MORE PRONOUNCED IN MOUSE MODELS OF MUCOPOLYSACCHARIDOSIS (MPS) TYPE IIIA AND IIIB OVER MPSI. In: JOURNAL OF INHERITED METABOLIC DISEASE. pp.S22-S22.
ME. Alonso-Ferrero, A. Langford-Smith, D. Burke, S. Heales, BW. Bigger, et al. A. Thrasher, B. Gaspar. (2012). Gene Therapy for Lysosomal Storage Diseases using an enhanced lentiviral vector delivery system. In: HUMAN GENE THERAPY. UCL Inst Child Hlth, London, ENGLAND, 9/3/2012. pp.A14-A14.
A. Sergijenko, A. Langford-Smith, KJ. Langford-Smith, RF. Wynn (2012). Correction of the Mucopolysaccharidosis IIIA mouse via stem cell gene therapy using a CD11b promoter to improve myeloid specific expression in the brain. In: HUMAN GENE THERAPY. UCL Inst Child Hlth, London, ENGLAND, 9/3/2012. pp.A13-A13.
A. Sergijenko, A. Langford-Smith, K. Langford-Smith, R. Wynn, E. Wraith, et al. S. Jones, F. Wilkinson, B. Bigger. (2011). Improved lentiviral vectors for haematopoietic stem cell gene therapy of Mucopolysaccharidosis Type IIIA (MPSIIIA). In: HUMAN GENE THERAPY. pp.A52-A53.
A. Langford-Smith, F. Wilkinson, KJ. Langford-Smith, A. Sergijenko, W. Bennett, et al. S. Howe, A. Thrasher, S. Jones, JE. Wraith, R. Wynn, B. Bigger. (2011). Lentiviral vector enhanced haematopoietic stem cell gene therapy for mucopolysaccharidosis type IIIA. In: BONE MARROW TRANSPLANTATION. Paris, FRANCE, 3/4/2011. pp.S316-S316.
KJ. Langford-Smith, Z. Sandiford, A. Langford-Smith, F. Wilkinson, S. Jones, et al. JE. Wraith, R. Wynn, B. Bigger. (2011). An improved mouse model of haematopoietic stem cell transplantation for investigating reduced-intensity conditioning regimens. In: BONE MARROW TRANSPLANTATION. Paris, FRANCE, 3/4/2011. pp.S300-S300.
B. Bigger, A. Langford-Smith, F. Wilkinson, K. Langford-Smith, A. Sergijenko, et al. E. Wraith, R. Wynn. (2011). Lentiviral mediated stem cell gene therapy corrects a mouse model of mucopolysaccharidosis type IIIA. In: Molecular Genetics and Metabolism. Las Vegas, NV, 16/2/2011. pp.S8-S8.
A. Sergijenko, A. Langford-Smith, K. Langford-Smith, R. Wynn, E. Wraith, et al. F. Wilkinson, B. Bigger. (2010). Optimisation of Lentiviral Transduction Conditions of Haematopoietic Stem Cells for Treatment of Mucopolysaccharidosis Type IIIA (MPSIIIA). In: HUMAN GENE THERAPY. Royal Holloway Univ London, Egham, ENGLAND, 29/3/2010. pp.518-518.
AWW. Langford-Smith, FL. Wilkinson, KJ. Langford-Smith, W. Bennett, S. Howe, et al. A. Thrasher, K. Hemsley, J. Hopwood, JE. Wraith, RF. Wynn, BW. Bigger. (2010). Lentiviral Vector-Mediated Stem Cell Gene Therapy for Mucopolysaccharidosis Type IIIA (MPSIIIA). In: HUMAN GENE THERAPY. Royal Holloway Univ London, Egham, ENGLAND, 29/3/2010. pp.502-503.
B. Bigger, M. Malinowska, F. Wilkinson, K. Langford-Smith, A. Langford-Smith, et al. R. Wynn, E. Wraith, G. Wegrzyn, B. Bigger. (2010). 15. The effect of long-term substrate reduction therapy withgenistein in a mouse model of MPS IIIB. In: Molecular Genetics and Metabolism. Miami, FL, 10/2/2010. pp.S11-S11.
A. Langford-Smith, F. Wilkinson, W. Bennett, J. Hopwood, E. Wraith, et al. R. Wynn, B. Bigger. (2009). Lentiviral vector-mediated stem cell gene therapy for mucopolysaccharidosis type IIIA (MPSIIIA). In: HUMAN GENE THERAPY. Hannover, GERMANY, 21/11/2009. pp.1396-1397.
2013 Get Connected 3:ImmunoMatrix - The dynamic interplay between the immune system and the extracellular matrix
Oral presentations:
North West Vascular Meeting 2017 (1st prize)
Vascular Society 2017 (Finalist)
Biomedicine Showcase 2010 (1st prize)
Poster presentations:
Mercia Stem Cell Alliance Annual Scientific Meeting 2016 (2nd prize)
11th Keele Aluminium Meeting 2015 (2nd prize)