Dr Marilena Crescente
Lecturer in Haematology
My profile
Biography
BIOGRAPHY
Marilena is Lecturer of Haematology at Manchester Metropolitan and an Honorary Research Fellow at Queen Mary University of London. Since completing her PhD in Vascular Biology and Pathophysiology at the University of Perugia, she has worked in several international Academic labs where she has built a successful research career studying platelet biology and pharmacology of anti-platelet drugs. Marilena worked in the laboratory of Professor Denisa Wagner at the Harvard Medical School in Boston, where she characterised the use of recombinant human ADAMTS13 and tPA-coated nanoparticles as thrombolytic drugs. This work was partially supported by a fellowship from the Foundation BLANCEFLOR Boncompagni-Ludovisi. She then worked with Dr Alicia Garcia Arroyo in Madrid at the CNIC (Spanish National Center for Cardiovascular Research) studying the involvement of platelet matrix metalloproteinases in angiogenesis. Since moving to the UK in 2012, she worked at the University of Reading with Professor Jonathan Gibbins to study the biogenesis of platelet thiol isomerases. Subsequently she moved to Queen Mary University of London where in Tim Warner’s lab she characterised the eicosanoid profiles underlying the anti- and pro-thrombotic actions of aspirin in the cardiovascular system.
Marilena is now establishing her own laboratory at Manchester Metropolitan. Her main research is on the role of platelet-derived lipid mediators in host immune response to fungal pathogens, especially Candida albicans and Aspergillus fumigatus. This ambitious programme brings together platelet biology, immunology, lipidomics and bioinformatics and it has been funded by the prestigious Academy of Medical Sciences Springboard Award that Marilena has received in February 2022. To support her research, Marilena has forged several collaborations with mycology experts such as Dr Neil McCarthy (Queen Mary University of London) and Professor Katrien Lagrou (Katholieke Universiteit Leuven). Moreover, she collaborates with Professor Kimberly Martinod (Katholieke Universiteit Leuven) who is a worldwide expert on Neutrophil Extracellular Traps (NETs) biology.
ACADEMIC AND PROFESSIONAL QUALIFICATIONS
PhD University of Perugia (2010)
Associate Fellow HEA (2020)
Good Clinical Practice (November 2018)
Pharmacist Qualification (July 2005)
PROFESSIONAL ACTIVITIES
Peer review for Blood, British Journal of Pharmacology, Scientific Reports and Platelets
MEMBERSHIP OF PROFESSIONAL ASSOCIATIONS
Associate Fellow -Higher Education Academy
Member of the International Society on Thrombosis and Haemostasis
Member of the Platelet Society
Member of the British Pharmacological Society
Interests and expertise
I teach Haematology to undergraduate and postgraduate students.
I’m a scientist and my research brings together platelet biology, immunology and microbiology with the aim to find platelet-mediated immune mechanisms regulating host response to fungal pathogens. For this purpose I use different approaches including flow cytometry, confocal microscopy, lipidomics and bioinformatics.
Projects
My lab is externally supported by the Academy of Medical Sciences and receives internal funding from Manchester Metropolitan University.
Self-funded MRes and PhD students are encouraged to get in touch to discuss potential project opportunities.
Teaching
UNDERGRADUATE TEACHING
BSC BIOMEDICAL SCIENCE
Haematology - Unit: Blood Science
POSTGRADUATE TEACHING
MSc Biomedical Sciences and STP Clinical Sciences in Haematology and Transfusion
Haematology Unit: Clinical Haematology
Supervision
Postgraduate Research
Supervision of MRes Students
Self-funded MRes and PhD students are encouraged to get in touch to discuss potential project opportunities.
Research outputs
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Journal articles
Martinod, K., Denorme, F., Meyers, S., Crescente, M., Van Bruggen, S., Stroobants, M., Siegel, P.M., Grandhi, R., Glatz, K., Witsch, T. (2024) 'Involvement of peptidylarginine deiminase 4 in eosinophil extracellular trap formation and contribution to citrullinated histone signal in thrombi.' Journal of Thrombosis and Haemostasis,
Kellett, D.O., Aziz, Q., Humphries, J.D., Korsak, A., Braga, A., Gutierrez Del Arroyo, A., Crescente, M., Tinker, A., Ackland, G.L., Gourine, A.V. (2024) 'Transcriptional response of the heart to vagus nerve stimulation.' Physiological Genomics,
Ferreira, P., Vaja, R., Lopes-Pires, M., Crescente, M., Yu, H., Nüsing, R., Liu, B., Zhou, Y., Yaqoob, M., Zhang, A., Rickman, M., Longhurst, H., White, W.E., Knowles, R.B., Chan, M.V., Warner, T.D., Want, E., Kirkby, N.S., Mitchell, J.A. (2023) 'Renal function underpins the cyclooxygenase-2: asymmetric dimethylarginine axis in mouse and man.' Kidney International Reports, 8(6) pp. 1231-1238.
Meyers, S., Lox, M., Kraisin, S., Liesenborghs, L., Martens, C.P., Frederix, L., Van Bruggen, S., Crescente, M., Missiakas, D., Baatsen, P., Vanassche, T., Verhamme, P., Martinod, K. (2023) 'Neutrophils Protect Against Staphylococcus aureus Endocarditis Progression Independent of Extracellular Trap Release.' Arteriosclerosis, Thrombosis and Vascular Biology, 43(2) pp. 267-285.
Cappelletto, A., Allan, H.E., Crescente, M., Schneider, E., Bussani, R., Ali, H., Secco, I., Vodret, S., Simeone, R., Mascaretti, L., Zacchigna, S., Warner, T.D., Giacca, M. (2023) 'SARS-CoV-2 Spike protein activates TMEM16F-mediated platelet procoagulant activity.' Frontiers in Cardiovascular Medicine, 9pp. 1013262-1013262.
Armstrong, P.C., Allan, H.E., Kirkby, N.S., Gutmann, C., Joshi, A., Crescente, M., Mitchell, J.A., Mayr, M., Warner, T.D. (2022) 'Temporal in vivo platelet labeling in mice reveals age-dependent receptor expression and conservation of specific mRNAs.' Blood Advances, 6(23) pp. 6028-6038.
Hsu, H., Chan, M.V., Armstrong, P.C., Crescente, M., Donikian, D., Kondo, M., Brighton, T., Chen, V., Chen, Q., Connor, D., Joseph, J., Morel-Kopp, M.C., Stevenson, W.S., Ward, C., Warner, T.D., Rabbolini, D.J. (2022) 'A pilot study assessing the implementation of 96-well plate-based aggregometry (Optimul) in Australia.' Pathology, 54(6) pp. 746-754.
Meyers, S., Crescente, M., Verhamme, P., Martinod, K. (2022) 'Staphylococcus aureus and Neutrophil Extracellular Traps: the Master Manipulator Meets its Match in Immunothrombosis.' Arteriosclerosis, Thrombosis and Vascular Biology, 42(3) pp. 261-276.
Allan, H.E., Hayman, M.A., Marcone, S., Chan, M.V., Edin, M.L., Maffucci, T., Joshi, A., Menke, L., Crescente, M., Mayr, M., Zeldin, D.C., Armstrong, P.C., Warner, T.D. (2021) 'Proteome and functional decline as platelets age in the circulation.' Journal of Thrombosis and Haemostasis, 19(12) pp. 3095-3112.
Heywood, W.E., Bliss, E., Bahelil, F., Cyrus, T., Crescente, M., Jones, T., Iqbal, S., Paredes, L.G., Toner, A.J., del Arroyo, A.G., O'Toole, E.A., Mills, K., Ackland, G.L. (2021) 'Proteomic signatures for perioperative oxygen delivery in skin after major elective surgery: mechanistic sub-study of a randomised controlled trial.' British Journal of Anaesthesia, 127(4) pp. 511-520.
Mitchell, J.A., Kirkby, N.S., Ahmetaj-Shala, B., Armstrong, P.C., Crescente, M., Ferreira, P., Lopes Pires, M.E., Vaja, R., Warner, T.D. (2021) 'Cyclooxygenases and the cardiovascular system..' Pharmacology and Therapeutics, 217pp. 107624-107624.
Palma-Barqueros, V., Crescente, M., de la Morena, M.E., Chan, M.V., Almarza, E., Revilla, N., Bohdan, N., Miñano, A., Padilla, J., Allan, H.E., Maffucci, T., Edin, M.L., Zeldin, D.C., Mesa-Nuñez, C., Damian, C., Marín-Quilez, A., Benito, R., Martínez-Martínez, I., Bermejo, N., Casas-Aviles, I., Rodríguez-Alen, A., González-Porras, J.R., Hernández-Rivas, J.M., Vicente, V., Corral, J., Lozano, M.L., Warner, T.D., Bastida, J.M., Rivera, J. (2020) 'A novel genetic variant in PTGS1 affects N-glycosylation of cyclooxygenase-1 causing a dominant-negative effect on platelet function and bleeding diathesis..' American Journal of Hematology, 96(3) pp. E83-E88.
Sahli, K., Flora, G., Sasikumar, P., Maghrabi, A., Holbrook, L., AlOuda, S., Elgheznawy, A., Sage, T., Stainer, A., Adiyaman, R., AboHassan, M., Crescente, M., Kriek, N., Vayiyapuri, S., Bye, A., Unsworth, A., Jones, C., McGuffin, L., Gibbins, J. (2020) 'Structural, Functional and Mechanistic Insights Uncover the Fundamental Role of Orphan Connexin-62 in Platelets.' Blood, 137(6) pp. 830-843.
Crescente, M., Armstrong, P.C., Kirkby, N.S., Edin, M.L., Chan, M.V., Lih, F.B., Jiao, J., Maffucci, T., Allan, H.E., Mein, C.A., Gaston-Massuet, C., Cottrell, G.S., Mitchell, J.A., Zeldin, D.C., Herschman, H.R., Warner, T.D. (2020) 'Profiling the eicosanoid networks that underlie the anti- and pro-thrombotic effects of aspirin..' The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(8) pp. 10027-10040.
Chan, M.V., Hayman, M.A., Sivapalaratnam, S., Crescente, M., Allan, H.E., Edin, M.L., Zeldin, D.C., Milne, G.L., Stephens, J., Greene, D., Hanif, M., O'Donnell, V.B., Dong, L., Malkowski, M.G., Lentaigne, C., Wedderburn, K., Stubbs, M., Downes, K., Ouwehand, W.H., Turro, E., NIHR BioResource, , Hart, D.P., Freson, K., Laffan, M.A., Warner, T.D. (2020) 'Identification of a homozygous recessive variant in PTGS1 resulting in a congenital aspirin-like defect in platelet function.' Haematologic, 106(5) pp. 1423-1432.
Armstrong, P.C., Ferreira, P.M., Chan, M.V., Lundberg Slingsby, M.H., Crescente, M., Shih, C.C., Kirkby, N.S., Hobbs, A.J., Warner, T.D. (2020) 'Combination of cyclic nucleotide modulators with P2Y12 receptor antagonists as anti-platelet therapy.' Journal of Thrombosis and Haemostasis, 18(7) pp. 1705-1713.
Flora, G.D., Sahli, K.A., Sasikumar, P., Holbrook, L.M., Stainer, A.R., AlOuda, S.K., Crescente, M., Sage, T., Unsworth, A.J., Gibbins, J.M. (2019) 'Non-genomic effects of the Pregnane X Receptor negatively regulate platelet functions, thrombosis and haemostasis.' Scientific Reports, 9
Izzi, B., Gianfagna, F., Yang, W.Y., Cludts, K., De Curtis, A., Verhamme, P., Di Castelnuovo, A., Cerletti, C., Donati, M.B., de Gaetano, G., Staessen, J.A., Hoylaerts, M.F., Iacoviello, L., Moli-family Investigators, (2019) 'Variation of PEAR1 DNA methylation influences platelet and leukocyte function..' Clinical Epigenetics, 11(1) pp. 151-151.
Mitchell, J.A., Shala, F., Elghazouli, Y., Warner, T.D., Gaston-Massuet, C., Crescente, M., Armstrong, P.C., Herschman, H.R., Kirkby, N.S. (2019) 'Cell-Specific Gene Deletion Reveals the Antithrombotic Function of COX1 and Explains the Vascular COX1/Prostacyclin Paradox..' Circulation Research, 125(9) pp. 847-854.
Noro, F., Gianfagna, F., Gialluisi, A., De Curtis, A., Di Castelnuovo, A., Napoleone, E., Cerletti, C., Donati, M.B., de Gaetano, G., Hoylaerts, M.F., Iacoviello, L., Izzi, B., Moli-family study Investigators, (2019) 'ZBTB12 DNA methylation is associated with coagulation- and inflammation-related blood cell parameters: findings from the Moli-family cohort..' Clinical Epigenetics, 11(1) pp. 74-74.
Crescente, M., Menke, L., Chan, M.V., Armstrong, P.C., Warner, T.D. (2018) 'Eicosanoids in platelets and the effect of their modulation by aspirin in the cardiovascular system (and beyond).' British Journal of Pharmacology, 176(8) pp. 988-999.
Unsworth, A.J., Bye, A.P., Tannetta, D.S., Desborough, M.J.R., Kriek, N., Sage, T., Allan, H.E., Crescente, M., Yaqoob, P., Warner, T.D., Jones, C.I., Gibbins, J.M. (2017) 'Farnesoid X Receptor and Liver X Receptor Ligands Initiate Formation of Coated Platelets..' Arteriosclerosis, Thrombosis, and Vascular Biology, 37(8) pp. 1482-1493.
Unsworth, A.J., Flora, G.D., Sasikumar, P., Bye, A.P., Sage, T., Kriek, N., Crescente, M., Gibbins, J.M. (2017) 'RXR Ligands Negatively Regulate Thrombosis and Hemostasis..' Arteriosclerosis, Thrombosis, and Vascular Biology, 37(5) pp. 812-822.
Crescente, M., Pluthero, F.G., Li, L., Lo, R.W., Walsh, T.G., Schenk, M.P., Holbrook, L.M., Louriero, S., Ali, M.S., Vaiyapuri, S., Falet, H., Jones, I.M., Poole, A.W., Kahr, W.H.A., Gibbins, J.M. (2016) 'Intracellular trafficking, localization, and mobilization of platelet-borne thiol isomerases.' Arteriosclerosis, Thrombosis, and Vascular Biology, 36(6) pp. 1164-1173.
Fantacuzzi, M., Giampietro, L., Crescente, M. (2016) 'Synthetic approach to phenylalanine-proline, and tyrosine-proline hybrid amino acids.' Current Bioactive Compounds, 12(3) pp. 168-181.
Vaiyapuri, S., Roweth, H., Ali, M.S., Unsworth, A.J., Stainer, A.R., Flora, G.D., Crescente, M., Jones, C.I., Moraes, L.A., Gibbins, J.M. (2015) 'Pharmacological actions of nobiletin in the modulation of platelet function.' British Journal of Pharmacology, 172(16) pp. 4133-4145.
Gianfagna, F., Tamburrelli, C., Vohnout, B., Crescente, M., Izzi, B., Pampuch, A., De Curtis, A., Di Castelnuovo, A., Cutrone, A., Napoleone, E., Tayo, B., Lorenzet, R., Nanni, L., Arca, M., Donati, M.B., de Gaetano, G., Cerletti, C., Iacoviello, L. (2013) 'Heritability, genetic correlation and linkageto the 9p21.3 region of mixed platelet-leukocyte conjugates in families with and without early myocardial infarction.' Nutrition, Metabolism and Cardiovascular Diseases, 23(7) pp. 684-692.
Crescente, M., Thomas, G.M., Demers, M., Voorhees, J.R., Wong, S.L., Ho-Tin-Noe, B., Wagner, D.D. (2012) 'ADAMTS13 exerts a thrombolytic effect in microcirculation.' Thrombosis and Haemostasis, 108(3) pp. 527-532.
Korin, N., Kanapathipillai, M., Matthews, B.D., Crescente, M., Brill, A., Mammoto, T., Ghosh, K., Jurek, S., Bencherif, S.A., Bhatta, D., Coskun, A.U., Feldman, C.L., Wagner, D.D., Ingber, D.E. (2012) 'Shear-activated nanotherapeutics for drug targeting to obstructed blood vessels.' Science, 337(6095) pp. 738-742.
Giordano, L., Coletta, W., Tamburrelli, C., D'Imperio, M., Crescente, M., Silvestri, C., Rapisarda, P., Reforgiato Recupero, G., De Curtis, A., Iacoviello, L., De Gaetano, G., Rotilio, D., Cerletti, C., Donati, M.B. (2012) 'Four-week ingestion of blood orange juice results in measurable anthocyanin urinary levels but does not affect cellular markers related to cardiovascular risk: A randomized cross-over study in healthy volunteers.' European Journal of Nutrition, 51(5) pp. 541-548.
Della Corte, A., Tamburrelli, C., Crescente, M., Giordano, L., D'Imperio, M., Di Michele, M., Donati, M.B., De Gaetano, G., Rotilio, D., Cerletti, C. (2012) 'Platelet proteome in healthy volunteers who smoke.' Platelets, 23(2) pp. 91-105.
Tamburrelli, C., Crescente, M., Izzi, B., Barisciano, M., Donati, M.B., De Gaetano, G., Cerletti, C. (2011) 'Epoprostenol inhibits human platelet-leukocyte mixed conjugate and platelet microparticle formation in whole blood.' Thrombosis Research, 128(5) pp. 446-451.
Vohnout, B., Gianfagna, F., Lorenzet, R., Cerletti, C., de Gaetano, G., Donati, M.B., Iacoviello, L. (2011) 'Genetic regulation of inflammation-mediated activation of haemostasis: Family-based approaches in population studies.' NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES, 21(11) pp. 857-861.
Crescente, M., Mezzasoma, A.M., Del Pinto, M., Palmerini, F., Di Castelnuovo, A., Cerletti, C., De Gaetano, G., Gresele, P. (2011) 'Incomplete inhibition of platelet function as assessed by the platelet function analyzer (PFA-100) identifies a subset of cardiovascular patients with high residual platelet response while on aspirin.' Platelets, 22(3) pp. 179-187.
Krishnan, S., Gaspari, M., Corte, A.D., Bianchi, P., Crescente, M., Cerletti, C., Torella, D., Indolfi, C., de Gaetano, G., Donati, M.B., Rotilio, D., Cuda, G. (2011) 'OFFgel-based multidimensional LC-MS/MS approach to the cataloguing of the human platelet proteome for an interactomic profile.' Electrophoresis, 32(6-7) pp. 686-695.
Crescente, M., Jessen, G., Momi, S., Höltje, H.D., Gresele, P., Cerletti, C., De Gaetano, G. (2009) 'Interactions of gallic acid, resveratrol, quercetin and aspirin at the platelet cyclooxygenase-1 level: Functional and modelling studies.' Thrombosis and Haemostasis, 102(2) pp. 336-346.
De Gaetano, G., Crescente, M., Cerletti, C. (2008) 'Current concepts about inhibition of platelet aggregation.' Platelets, 19(8) pp. 565-570.
Crescente, M., Di Castelnuovo, A., Iacoviello, L., De Gaetano, G., Cerletti, C. (2008) 'PFA-100 closure time to predict cardiovascular events in aspirin-treated cardiovascular patients: A meta-analysis of 19 studies comprising 3,003 patients.' Thrombosis and Haemostasis, 99(6) pp. 1129-1131.
Crescente, M., Di Castelnuovo, A., Iacoviello, L., De Gaetano, G., Cerletti, C. (2008) 'Rebuttal to "Aspirin response variability assessed with the PFA-100 device" by Reny et al.' Thrombosis and Haemostasis, 99(5)
Crescente, M., Di Castelnuovo, A., Iacoviello, L., Vermylen, J., Cerletti, C., de Gaetano, G. (2008) 'Response variability to aspirin as assessed by the platelet function analyzer (PFA)-100. A systematic review.' Thrombosis and Haemostasis, 99(1) pp. 14-26.
Crescente, M., Cerletti, C., de Gaetano, G. (2007) 'Gallic acid, a dietary polyphenolic components, blunts the inhibition of platelet COX-1 by aspirin: Preliminary in-vitro findings [4].' Thrombosis and Haemostasis, 97(6) pp. 1054-1056.